Skip to content

Home
Research themes
Publications
News and events
People
SmartTools
Contact
Toggle website search

Home
Research themes
Publications
News and events
People
SmartTools
Contact
Toggle website search

Loading view.

There were no results found.

There were no results found.

Lab Meeting

Events
Lab Meeting

Views Navigation

Event Views Navigation

List
Month
Day

Select date.

Previous Events
Today

Google Calendar
iCalendar
Outlook 365
Outlook Live
Export .ics file
Export Outlook .ics file

Recent Posts

20240415_membrane digest
20240408_membrane digest
20240401_membrane digest
20240325_membrane digest
20240315_membrane digest

Upcoming Events

There are no upcoming events.

Categories

Categories

Last publications

2503009 63TU6NVS 2024 items 1 3 date desc 5260 http://umr7099.ibpc.fr/wp-content/plugins/zotpress/

%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-80a3f354e72417c02b188c8e9786eca9%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%229M3UKART%22%2C%22library%22%3A%7B%22id%22%3A2503009%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zuniga%20et%20al.%22%2C%22parsedDate%22%3A%222024-02-12%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EZuniga%2C%20D.%2C%20Zoumpoulakis%2C%20A.%2C%20Veloso%2C%20R.F.%2C%20Peverini%2C%20L.%2C%20Shi%2C%20S.%2C%20Pozza%2C%20A.%2C%20Kugler%2C%20V.%2C%20Bonnet%26%23xE9%3B%2C%20F.%2C%20Bouceba%2C%20T.%2C%20Wagner%2C%20R.%2C%20Corringer%2C%20P.-J.%2C%20Fernandes%2C%20C.A.H.%2C%20V%26%23xE9%3Bnien-Bryan%2C%20C.%2C%202024.%20Biochemical%2C%20biophysical%2C%20and%20structural%20investigations%20of%20two%20mutants%20%28C154Y%20and%20R312H%29%20of%20the%20human%20Kir2.1%20channel%20involved%20in%20the%20Andersen-Tawil%20syndrome.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1101%5C%2F2024.02.09.579451%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1101%5C%2F2024.02.09.579451%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22preprint%22%2C%22title%22%3A%22Biochemical%2C%20biophysical%2C%20and%20structural%20investigations%20of%20two%20mutants%20%28C154Y%20and%20R312H%29%20of%20the%20human%20Kir2.1%20channel%20involved%20in%20the%20Andersen-Tawil%20syndrome%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dania%22%2C%22lastName%22%3A%22Zuniga%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreas%22%2C%22lastName%22%3A%22Zoumpoulakis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rafael%20F.%22%2C%22lastName%22%3A%22Veloso%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laurie%22%2C%22lastName%22%3A%22Peverini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophie%22%2C%22lastName%22%3A%22Shi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandre%22%2C%22lastName%22%3A%22Pozza%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Val%5Cu00e9rie%22%2C%22lastName%22%3A%22Kugler%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7oise%22%2C%22lastName%22%3A%22Bonnet%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tahar%22%2C%22lastName%22%3A%22Bouceba%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Renaud%22%2C%22lastName%22%3A%22Wagner%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre-Jean%22%2C%22lastName%22%3A%22Corringer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carlos%20A.%20H.%22%2C%22lastName%22%3A%22Fernandes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Catherine%22%2C%22lastName%22%3A%22V%5Cu00e9nien-Bryan%22%7D%5D%2C%22abstractNote%22%3A%22Inwardly%20rectifying%20potassium%20%28Kir%29%20channels%20play%20a%20pivotal%20role%20in%20physiology%20by%20establishing%2C%20maintaining%2C%20and%20regulating%20the%20resting%20membrane%20potential%20of%20the%20cells%2C%20particularly%20contributing%20to%20the%20cellular%20repolarization%20of%20many%20excitable%20cells.%20Dysfunction%20in%20Kir2.1%20channels%20is%20implicated%20in%20several%20chronic%20and%20debilitating%20human%20diseases%20for%20which%20there%20are%20currently%20no%20effective%20treatments.%20Specifically%2C%20Kir2.1-R312H%20and%20Kir2.1-C154Y%20mutations%20are%20associated%20with%20Andersen-Tawil%20syndrome%20%28ATS%29%20in%20humans.%20We%20have%20performed%20structural%20studies%20and%20have%20investigated%20the%20impact%20of%20these%20two%20mutants%20in%20the%20trafficking%20of%20the%20channel%20to%20the%20cell%20membrane%20and%20function%20in%20Xenopus%20laevis%20oocytes.%20Despite%20both%20mutations%20being%20successfully%20trafficked%20to%20the%20cell%20membrane%20and%20capable%20of%20binding%20PIP2%20%28phosphatidylinositol-4%2C5-bisphosphate%29%2C%20the%20main%20modulator%20for%20channel%20activity%2C%20they%20resulted%20in%20defective%20channels%20that%20do%20not%20display%20K%2B%20current%2C%20albeit%20through%20different%20molecular%20mechanisms.%20Co-expression%20studies%20showed%20that%20R312H%20and%20C154Y%20are%20expressed%20and%20associated%20with%20the%20WT%20subunits.%20While%20WT%20subunits%20could%20rescue%20R312H%20dysfunction%2C%20the%20presence%20of%20a%20unique%20C154Y%20subunit%20disrupts%20the%20function%20of%20the%20entire%20complex%2C%20which%20is%20a%20typical%20feature%20of%20mutations%20with%20a%20dominant-negative%20effect.%20Molecular%20dynamics%20simulations%20showed%20that%20Kir2.1-C154Y%20mutation%20induces%20a%20loss%20in%20the%20structural%20plasticity%20of%20the%20selectivity%20filter%2C%20impairing%20the%20K%2B%20flow.%20Furthermore%2C%20the%20cryo-EM%20structure%20of%20the%20Kir2.1-R312H%20mutant%20cytoplasmic%20region%20confirmed%20previous%20findings%20that%20this%20mutation%20leads%20to%20a%20loss%20of%20the%20inter-subunit%20interaction%20network%20between%20residues%20R312%2C%20H221%2C%20and%20E303%2C%20thereby%20impairing%20channel%20gating%20at%20the%20G-loop%20level.%20Therefore%2C%20this%20study%20identified%20the%20molecular%20mechanisms%20by%20which%20two%20ATS-causing%20mutations%20impact%20Kir2.1%20channel%20function%20and%20provide%20valuable%20insights%20that%20can%20guide%20potential%20strategies%20for%20the%20development%20of%20future%20therapeutic%20interventions%20for%20ATS.%22%2C%22genre%22%3A%22%22%2C%22repository%22%3A%22bioRxiv%22%2C%22archiveID%22%3A%22%22%2C%22date%22%3A%222024-02-12%22%2C%22DOI%22%3A%2210.1101%5C%2F2024.02.09.579451%22%2C%22citationKey%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.biorxiv.org%5C%2Fcontent%5C%2F10.1101%5C%2F2024.02.09.579451v1%22%2C%22language%22%3A%22en%22%2C%22collections%22%3A%5B%2263TU6NVS%22%5D%2C%22dateModified%22%3A%222024-02-23T12%3A45%3A03Z%22%7D%7D%2C%7B%22key%22%3A%22LLJNY6LJ%22%2C%22library%22%3A%7B%22id%22%3A2503009%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Adrien%20et%20al.%22%2C%22parsedDate%22%3A%222024-01-09%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAdrien%2C%20V.%2C%20Taulier%2C%20N.%2C%20Verch%26%23xE8%3Bre%2C%20A.%2C%20Monlezun%2C%20L.%2C%20Picard%2C%20M.%2C%20Ducruix%2C%20A.%2C%20Broutin%2C%20I.%2C%20Pincet%2C%20F.%2C%20Urbach%2C%20W.%2C%202024.%20Kinetic%20study%20of%20membrane%20protein%20interactions%3A%20from%20three%20to%20two%20dimensions.%20Sci%20Rep%2014%2C%20882.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-023-50827-5%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-023-50827-5%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Kinetic%20study%20of%20membrane%20protein%20interactions%3A%20from%20three%20to%20two%20dimensions%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vladimir%22%2C%22lastName%22%3A%22Adrien%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicolas%22%2C%22lastName%22%3A%22Taulier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alice%22%2C%22lastName%22%3A%22Verch%5Cu00e8re%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Monlezun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martin%22%2C%22lastName%22%3A%22Picard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Arnaud%22%2C%22lastName%22%3A%22Ducruix%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Isabelle%22%2C%22lastName%22%3A%22Broutin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fr%5Cu00e9d%5Cu00e9ric%22%2C%22lastName%22%3A%22Pincet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wladimir%22%2C%22lastName%22%3A%22Urbach%22%7D%5D%2C%22abstractNote%22%3A%22Molecular%20interactions%20are%20contingent%20upon%20the%20system%5Cu2019s%20dimensionality.%20Notably%2C%20comprehending%20the%20impact%20of%20dimensionality%20on%20protein%5Cu2013protein%20interactions%20holds%20paramount%20importance%20in%20foreseeing%20protein%20behaviour%20across%20diverse%20scenarios%2C%20encompassing%20both%20solution%20and%20membrane%20environments.%20Here%2C%20we%20unravel%20interactions%20among%20membrane%20proteins%20across%20various%20dimensionalities%20by%20quantifying%20their%20binding%20rates%20through%20fluorescence%20recovery%20experiments.%20Our%20findings%20are%20presented%20through%20the%20examination%20of%20two%20protein%20systems%3A%20streptavidin%5Cu2013biotin%20and%20a%20protein%20complex%20constituting%20a%20bacterial%20efflux%20pump.%20We%20present%20here%20an%20original%20approach%20for%20gauging%20a%20two-dimensional%20binding%20constant%20between%20membrane%20proteins%20embedded%20in%20two%20opposite%20membranes.%20The%20quotient%20of%20protein%20binding%20rates%20in%20solution%20and%20on%20the%20membrane%20represents%20a%20metric%20denoting%20the%20exploration%20distance%20of%20the%20interacting%20sites%5Cu2014a%20novel%20interpretation.%22%2C%22date%22%3A%222024-1-9%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-023-50827-5%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.ncbi.nlm.nih.gov%5C%2Fpmc%5C%2Farticles%5C%2FPMC10776792%5C%2F%22%2C%22collections%22%3A%5B%2263TU6NVS%22%5D%2C%22dateModified%22%3A%222024-01-12T15%3A02%3A10Z%22%7D%7D%2C%7B%22key%22%3A%22XJ7XYJ9P%22%2C%22library%22%3A%7B%22id%22%3A2503009%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zinke%20et%20al.%22%2C%22parsedDate%22%3A%222024-01-06%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%201.35%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EZinke%2C%20M.%2C%20Lejeune%2C%20M.%2C%20Mechaly%2C%20A.%2C%20Bardiaux%2C%20B.%2C%20Boneca%2C%20I.G.%2C%20Delepelaire%2C%20P.%2C%20Izadi-Pruneyre%2C%20N.%2C%202024.%20Ton%20motor%20conformational%20switch%20and%20peptidoglycan%20role%20in%26%23xA0%3Bbacterial%20nutrient%20uptake.%20Nat%20Commun%2015%2C%20331.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-023-44606-z%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-023-44606-z%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Ton%20motor%20conformational%20switch%20and%20peptidoglycan%20role%20in%5Cu00a0bacterial%20nutrient%20uptake%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maximilian%22%2C%22lastName%22%3A%22Zinke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maylis%22%2C%22lastName%22%3A%22Lejeune%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ariel%22%2C%22lastName%22%3A%22Mechaly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benjamin%22%2C%22lastName%22%3A%22Bardiaux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivo%20Gomperts%22%2C%22lastName%22%3A%22Boneca%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philippe%22%2C%22lastName%22%3A%22Delepelaire%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nadia%22%2C%22lastName%22%3A%22Izadi-Pruneyre%22%7D%5D%2C%22abstractNote%22%3A%22Active%20nutrient%20uptake%20is%20fundamental%20for%20survival%20and%20pathogenicity%20of%20Gram-negative%20bacteria%2C%20which%20operate%20a%20multi-protein%20Ton%20system%20to%20transport%20essential%20nutrients%20like%20metals%20and%20vitamins.%20This%20system%20harnesses%20the%20proton%20motive%20force%20at%20the%20inner%20membrane%20to%20energize%20the%20import%20through%20the%20outer%20membrane%2C%20but%20the%20mechanism%20of%20energy%20transfer%20remains%20enigmatic.%20Here%2C%20we%20study%20the%20periplasmic%20domain%20of%20ExbD%2C%20a%20crucial%20component%20of%20the%20proton%20channel%20of%20the%20Ton%20system.%20We%20show%20that%20this%20domain%20is%20a%20dynamic%20dimer%20switching%20between%20two%20conformations%20representing%20the%20proton%20channel%27s%20open%20and%20closed%20states.%20By%20in%20vivo%20phenotypic%20assays%20we%20demonstrate%20that%20this%20conformational%20switch%20is%20essential%20for%20the%20nutrient%20uptake%20by%20bacteria.%20The%20open%20state%20of%20ExbD%20triggers%20a%20disorder%20to%20order%20transition%20of%20TonB%2C%20enabling%20TonB%20to%20supply%20energy%20to%20the%20nutrient%20transporter.%20We%20also%20reveal%20the%20anchoring%20role%20of%20the%20peptidoglycan%20layer%20in%20this%20mechanism.%20Herein%2C%20we%20propose%20a%20mechanistic%20model%20for%20the%20Ton%20system%2C%20emphasizing%20ExbD%20duality%20and%20the%20pivotal%20catalytic%20role%20of%20peptidoglycan.%20Sequence%20analysis%20suggests%20that%20this%20mechanism%20is%20conserved%20in%20other%20systems%20energizing%20gliding%20motility%20and%20membrane%20integrity.%20Our%20study%20fills%20important%20gaps%20in%20understanding%20bacterial%20motor%20mechanism%20and%20proposes%20novel%20antibacterial%20strategies.%22%2C%22date%22%3A%222024-01-06%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-023-44606-z%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%2263TU6NVS%22%5D%2C%22dateModified%22%3A%222024-01-12T14%3A53%3A22Z%22%7D%7D%5D%7D

Zuniga, D., Zoumpoulakis, A., Veloso, R.F., Peverini, L., Shi, S., Pozza, A., Kugler, V., Bonneté, F., Bouceba, T., Wagner, R., Corringer, P.-J., Fernandes, C.A.H., Vénien-Bryan, C., 2024. Biochemical, biophysical, and structural investigations of two mutants (C154Y and R312H) of the human Kir2.1 channel involved in the Andersen-Tawil syndrome. https://doi.org/10.1101/2024.02.09.579451

Adrien, V., Taulier, N., Verchère, A., Monlezun, L., Picard, M., Ducruix, A., Broutin, I., Pincet, F., Urbach, W., 2024. Kinetic study of membrane protein interactions: from three to two dimensions. Sci Rep 14, 882. https://doi.org/10.1038/s41598-023-50827-5

Zinke, M., Lejeune, M., Mechaly, A., Bardiaux, B., Boneca, I.G., Delepelaire, P., Izadi-Pruneyre, N., 2024. Ton motor conformational switch and peptidoglycan role in bacterial nutrient uptake. Nat Commun 15, 331. https://doi.org/10.1038/s41467-023-44606-z

Membranes & Molecules Seminars
Upcoming events

Copyright 2024 - Membrane Protein Biochemistry Lab