MP
Structure-based discovery of positive allosteric modulators of the A1 adenosine receptor.
Nguyen ATN, Panel N, Vo DD, Thai BS, Chia LY, Lu CS, Hellyer SD, Langiu M, Jörg M, Gregory KJ, Kihlberg J, White PJ, Scammells PJ, Christopoulos A, Carlsson J, May LT.
Proc Natl Acad Sci U S A. 2025 Jul 15;122(28):e2421687122.
doi: 10.1073/pnas.2421687122. Epub 2025 Jul 7.
PMID: 40623180.
P-type ATPase magnesium transporter MgtA acts as a dimer.
Zeinert R, Zhou F, Franco P, Zöller J, Madni ZK, Lessen H, Aravind L, Langer JD, Sodt AJ, Storz G, Matthies D.
Nat Struct Mol Biol. 2025 Jun 23.
doi: 10.1038/s41594-025-01593-7. Epub ahead of print.
PMID: 40550995.
Dimerization and substrate recognition of human taurine transporter.
Zhang Y, Chen J, Chen N, Xiong H, Zhu Z, Yang D, Ge J, Yu J.
Nat Commun. 2025 Jul 4;16(1):6163.
doi: 10.1038/s41467-025-60967-z.
PMID: 40615403.
Molecular basis for substrate recognition and transport of mammalian taurine transporters.
Wang M, He J, Cai Q, Zhang SA, She J.
Proc Natl Acad Sci U S A. 2025 Jul 8;122(27):e2425549122.
doi: 10.1073/pnas.2425549122. Epub 2025 Jul 2.
PMID: 40601627.
Evolutionary trajectory and functional diversity of SWEET sugar transporters in plants.
Li X, Ren Z, Zhang Z, Liu Y, He K, Zhang F, Guo J, Wei S, Yang D, Li W.
Plant J. 2025 Jul;123(1):e70334.
doi: 10.1111/tpj.70334.
PMID: 40628238.
Mutations in the proximal binding site and F-loop of AdeJ confer resistance to efflux pump inhibitors.
Saral Sariyer A, Leus IV, Tambat R, Farjana M, Olvera M, Rukmani SJ, Sariyer E, Smith JC, Parks JM, Walker JK, Zgurskaya HI.
Antimicrob Agents Chemother. 2025 Jul 8:e0009025.
doi: 10.1128/aac.00090-25. Online ahead of print.
PMID: 40626889.
The antiport mechanism of the human LAT1 transporter: defining the molecular determinants by means of wet and dry approaches.
Scalise M, Scanga R, Console L, Galluccio M, Indiveri C.
Biochem Biophys Res Commun. 2025 Jun 28;777:152277.
doi: 10.1016/j.bbrc.2025.152277. Online ahead of print.
PMID: 40616981.
At last: the mitochondrial pyruvate carrier structure revealed!
Finck BN, Hadfield CM, McCommis KS.
Trends Pharmacol Sci. 2025 Jul;46(7):596-598. doi: 10.1016/j.tips.2025.05.010. Epub 2025 Jun 10.
PMID: 40500675.
Membrane Transport of Rhodamine 6G by the reconstituted multidrug resistance ABC transporter Pdr5 in Horizontal Lipid Bilayer.
Daniel Blum, Lea Marie Nentwig, Stefanie L Gala Marti, Lutz Schmitt, and Richard Wagner.
bioRxiv posted 2 August 2025.
doi:10.1101/2025.07.31.667618.
A porin-like protein used by bacterial predators defines a wider lipid-trapping superfamily.
Parr RJ, Santin YG, Ratkevičiūte G, Caulton SG, Radford P, Gurvič D, Jenkins M, Doyle MT, Mead L, Silale A, van den Berg B, Knowles TJ, Sockett RE, Stansfeld PJ, Laloux G, Lovering AL.
Nat Commun. 2025 Jul 5;16(1):6213.
doi: 10.1038/s41467-025-61633-0.
PMID: 40617869.
Molecular determinants of substrate specificity in the efflux pump CraA from Acinetobacter baumannii.
Foong WE, Xiang X, Pos KM, Tam H-K.
Microbiol Spectr. 2025 Jul 7:e0111925.
doi: 10.1128/spectrum.01119-25. Online ahead of print.
PMID: 40621929.
The EMC acts as a chaperone for membrane proteins.
Klose CJ, Meighen-Berger KM, Kulke M, Parr M, Steigenberger B, Zacharias M, Frishman D, Feige MJ.
Nat Commun. 2025 Aug 2;16(1):7097.
doi: 10.1038/s41467-025-62109-x.
PMID: 40753078.
Function and firing of the Streptomyces coelicolor contractile injection system requires the membrane protein CisA.
Casu B, Sallmen JW, Haas PE, Chandra G, Afanasyev P, Xu J, Pilhofer M, Schlimpert S.
Elife. 2025 Jul 8;14:RP104064.
doi: 10.7554/eLife.104064.
PMID: 40626860.
Sodium-Potassium ATPase in cardiovascular diseases: Insights into structure, function, and therapeutic targets.
Leng B, Li Y, Wu G, Deng L.
Biochem Pharmacol. 2025 Jul 12:117163. doi: 10.1016/j.bcp.2025.117163. Online ahead of print.
PMID: 40659122.
Structural and functional analysis of the Mycobacterium tuberculosis MmpS5L5 efflux pump presages a pathway to increased bedaquiline resistance.
Fountain AJ, Böhning J, McLaughlin SH, Morgan TE, Edelstein PH, Troll M, Lamers MH, Bharat TAM, Luisi BF, Ramakrishnan L.
bioRxiv [Preprint]. 2025 Jun 24:2025.06.24.661325. doi: 10.1101/2025.06.24.661325.
PMID: 40667120.
Structures of MmpL complexes reveal the assembly and mechanism of this family of transporters.
Zhang Z, Maharjan R, Gregor WD, Klenotic PA, Yu EW.
bioRxiv [Preprint]. 2025 Jun 22:2025.06.22.660944.
doi: 10.1101/2025.06.22.660944.
PMID: 40667201.
Cryo-EM structures of a pentameric ligand-gated ion channel in liposomes.
Dalal V, Tan BK, Xu H, Cheng WWL.
Elife. 2025 Jul 16;14:RP106728.
doi: 10.7554/eLife.106728.
PMID: 40668221.
BioRxiv spotted in the 20250621’s edition of the Membrane’s Digest …
Design principles of the common Gly-X6-Gly membrane protein building block.
Golden K, Avarvarei C, Anderson CT, Holcomb M, Tang W, Dai X, Zhang M, Mailie CA, Sanchez BB, Chen JS, Forli S, Mravic M.
bioRxiv [Preprint]. 2025 Jun 27:2025.06.25.661420.
doi: 10.1101/2025.06.25.661420.
PMID: 40667231;
Membranes
The lipid bilayer strengthens the cooperative network of membrane proteins.
MuhammedNazaar S, Yao J, Necelis MR, Park YC, Shen Z, Bridges MD, Guo R, Swope N, Rhee MS, Kim M, Kim KH, Hubbell WL, Fleming KG, Columbus L, Kang SG, Hong H.
Sci Adv. 2025 Jul 4;11(27):eadv9568.
doi: 10.1126/sciadv.adv9568. Epub 2025 Jul 2.
PMID: 40601723.
Amino acids critical for lipid/s-interaction at the lipid-water-interface of TRPV5/TRPV6 remain different during vertebrate radiation: Relevance in cancer, bone disorders and other pathophysiologies.
Agarwal S, Kumar V, Chattapadhya A, Kumar S, Goswami L, Goswami C.
Biochim Biophys Acta Biomembr. 2025 Jun 30:184433. doi: 10.1016/j.bbamem.2025.184433. Online ahead of print.
PMID: 40602477.
Groove architecture controls lipid scrambling in simulations of protein and model systems.
Smith HE, Harrison-Rawn T, Zheng W, Ballesteros A, Sotomayor M.
bioRxiv [Preprint]. 2025 Jul 1:2025.06.27.662058.
doi: 10.1101/2025.06.27.662058.
PMID: 40631228.
Molecules
Detergent-resistant membranes in HeLa cells. A comparative study with an electrochemical and lipidomic perspective.
Mateckaja A, Zatloukalová M, Gonzalez G, Masař R, Najdekr L, Dostál Z, Dorčák V, Vacek J.
Chem Phys Lipids. 2025 Aug;270:105509.
doi: 10.1016/j.chemphyslip.2025.105509. Epub 2025 May 30.
PMID: 40451380.
Photoswitchable allosteric and dualsteric ligands in GPCR pharmacology.
Mori S, Arella D, Decker M.
Trends Pharmacol Sci. 2025 Jul 1:S0165-6147(25)00099-9. doi: 10.1016/j.tips.2025.05.009. Online ahead of print.
PMID: 40603191.
Methods
Evaluation of Small-Molecule Binding Site Prediction Methods on Membrane-Embedded Protein Interfaces.
Pliushcheuskaya P, Künze G.
J Chem Inf Model. 2025 Jul 2.
doi: 10.1021/acs.jcim.5c00336. Online ahead of print.
PMID: 40601846.
Review: Membrane protein nanodiscs for antibody discovery.
Yao X, Thomson CA.
Curr Opin Struct Biol. 2025 Jul 3;94:103104. doi: 10.1016/j.sbi.2025.103104. Online ahead of print.
PMID: 40614695.
Microbio
Structural and functional diversity of sensor domains in bacterial transmembrane receptors.
Matilla MA, Gavira JA, Monteagudo-Cascales E, Zhulin IB, Krell T.
Trends Microbiol. 2025 Jul;33(7):796-809.
doi: 10.1016/j.tim.2025.02.019. Epub 2025 Mar 21.
PMID: 40121131.
Contribution of RND superfamily multidrug efflux pumps AdeABC, AdeFGH, and AdeIJK to antimicrobial resistance and virulence factors in multidrug-resistant Acinetobacter baumannii AYE.
Xie L, Li J, Peng Q, Liu X, Lin F, Dai X, Ling B.
Antimicrob Agents Chemother. 2025 Jul 2;69(7):e0185824.
doi: 10.1128/aac.01858-24. Epub 2025 May 23.
PMID: 40407309.
Heterogeneous efflux pump expression underpins phenotypic resistance to antimicrobial peptides.
Lee KK, Łapińska U, Tolle G, Micaletto M, Zhang B, Phetsang W, Verderosa AD, Invergo BM, Westley J, Bebes A, Yuecel R, O’Neill PA, Farbos A, Jeffries AR, van Houte S, Caboni P, Blaskovich MAT, Housden BE, Tsaneva-Atanasova K, Pagliara S.
Elife. 2025 Jul 3;13:RP99752. doi: 10.7554/eLife.99752.
PMID: 40607907.
Wilkes RA, Miller TE, Waldbauer J, Zhou N, Zhang L, DiBiase BN, Kamat NP, Aristilde L, Beckham GT, Werner AZ.
Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida.
ACS Synth Biol. 2025 Jul 18;14(7):2739-2752.
doi: 10.1021/acssynbio.5c00171. Epub 2025 Jul 3.
PMID: 40607986.
Mechanisms of outer membrane vesicles in bacterial drug resistance: Insights and implications.
Zhang X, Ding W, Yang J, Gao L, Wang Q, Wang J, Luo Y, Yuan X, Sun B, Yang J, Zhou Y, Sun L.
Biochimie. 2025 Jul 23:S0300-9084(25)00165-8.
doi: 10.1016/j.biochi.2025.07.024. Online ahead of print.
PMID: 40712912.
Codon Usage and Antibiotic Resistance: A Hidden Evolutionary Mechanism.
Dahal U, Bansal A.
Biochimie. 2025 Jul 31:S0300-9084(25)00168-3.
doi: 10.1016/j.biochi.2025.07.027. Online ahead of print.
PMID: 40752602.
Rapid detection and capture of clinical Escherichia coli strains mediated by OmpA-targeting nanobodies.
Sorgenfrei M, Hürlimann LM, Printz A, Wegner F, Morger D, Ackle F, Remy MM, Montowski G, Keserue HA, Cuénod A, Imkamp F, Egli A, Keller PM, Seeger MA.
Commun Biol. 2025 Jul 14;8(1):1047. doi: 10.1038/s42003-025-08345-9.
Resistance in gram-negative bacilli: the emergence of RND efflux pumps.
Agreles MAA, de Moura ALP, Lima KFA, Aires CAM, de Almeida Campos LA, Cavalcanti IMF.
Future Microbiol. 2025 Jul 31:1-15.
doi: 10.1080/17460913.2025.2536375. Epub ahead of print.
PMID: 40742319.
Miscellaneous
DeepMind’s new AlphaGenome AI tackles the ‘dark matter’ in our DNA.
Callaway E.
Nature. 2025 Jul;643(8070):17-18.
doi: 10.1038/d41586-025-01998-w.
PMID: 40562910.